پالایش پساب آلوده به فنل بوسیله گونه ای از باکتری پانی باسیلوس

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکترای خاکشناسی، دانشگاه آزاد اسلامی، واحد خوراسگان

2 دانشیار گروه خاکشناسی، دانشگاه آزاد اسلامی، واحد خوراسگان

3 استادیارگروه میکرب شناسی، دانشگاه آزاد اسلامی، واحد خوراسگان

چکیده

فنل و ترکیبات آن برای محیط زیست حتی در مقادیر کم، موادی سمی و خطرناک محسوب می‌شوند لذا حذف این ترکیبات از پساب‌ها توجه محققان زیادی را به خود جلب کرده است. روشهای زیادی برای تجزیه فنل وجود دارد که بهترین روش، تصفیه زیستی است. در این تحقیق با هدف بررسی امکان وجود باکتری‌های تجزیه کننده فنل در پساب حاوی فنل اقدام به جداسازی جدایه‌های بومی تجزیه کننده این ترکیب، شناسایی جدایه برتر، بررسی روند رشد و حذف فنل به‌وسیله این جدایه و در نهایت شناسایی این جدایه با استفاده از روش واکنش زنجیری پلیمراز شد. نتایج نشان داد بهترین جدایه تجزیه کننده فنل گونه‌ای از پانی باسیلوس است که می‌تواند مقدار 1000 میلی‌گرم در لیتر فنل را در طی مدت زمان 96 ساعت به صفر برساند. نتایج این تحقیق تأکیدی بر استفاده باکتری‌های بومی برای حذف مقدار بالای فنل از محیط آلوده به این ترکیب است.

کلیدواژه‌ها

موضوعات


عنوان مقاله [English]

Reclamation of Phenol Contaminated Wastewater by Paenibacillus sp.

نویسندگان [English]

  • Somaye Eskandary 1
  • mehran Hoodaji 2
  • Arezoo Tahmoores pour 3
چکیده [English]

Phenol and its compounds are toxic and hazardous material for environment even in low concentrations. So removal of these compounds from wastewaters is very important. There are many methods for removal of phenol from contaminated wastewaters among which biodegradation is more attractive. In this study presuming the availabity of phenol degrading-bacterium in phenolic wastewater, indigenous bacterium were isolated and identified by PCR. The growth curve and phenol removal of this bacterium were prepared. Results showed that the best isolated was the stain of Paenibacillus that can remove 1000 mg.l-1 phenol during 96 hours. The results showed that indigenous bacterium can remove high concentration of phenol from contaminated wastewater.

کلیدواژه‌ها [English]

  • Polymerase Chain Reaction
  • isolation
  • Indigenous Bacterium
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